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  #1  
Old 01-08-2006, 01:19 PM
incredigirl incredigirl is offline
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Arrow Grade 11 Biology Project - Cryobiology/Freezing Tissue

I'm in grade 11 and really want to do a science fair project. I have been considering Cryobiology, like when they freeze animals and plants, but my problem is all I have been able to find is information on the subject nothing I could test or use. Could anybody help me by explaining a quick project idea on this topic, I'm open to anything!

Thanks!

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Old 01-08-2006, 02:09 PM
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Default Maybe...

This may sound wierd, but how about...

Seeing if freezing an animal actually preserves them intirely. Like maybe get a fish and freeze him in some water or something, then see by the end of a few weeks see if it is still perfectly preserved.
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Old 01-15-2006, 10:59 AM
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Okay, a few pointers...

you can't possibly freeze anything in water and expect them to be alive. The point of crybiology is you freeze the whole organism so quickly that the cells just immediately stop there. The reason why you can't freeze humans is because humans are too big, so humans would freeze slowly and they would die if the cells aren't frozen instantly. You have to use liquid nitrogen. Liquid nitrogen is colder than water, so the organism would freeze quickly. And the organism has to be small. Also, anything can't be frozen for too long. You can experiment with goldfish. (From experience, I have saw goldfish being frozen in liquid nitrogen and be revived by putting them quickly back into regular water. ) Check around how big a goldfish could be, and how long you can put the goldfish in liquid nitrogen, before you can't revive it anymore. Good luck!
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Old 01-22-2006, 07:48 PM
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Originally Posted by dreamerofeternity
Okay, a few pointers...

you can't possibly freeze anything in water and expect them to be alive. The point of crybiology is you freeze the whole organism so quickly that the cells just immediately stop there. The reason why you can't freeze humans is because humans are too big, so humans would freeze slowly and they would die if the cells aren't frozen instantly. You have to use liquid nitrogen. Liquid nitrogen is colder than water, so the organism would freeze quickly. And the organism has to be small. Also, anything can't be frozen for too long. You can experiment with goldfish. (From experience, I have saw goldfish being frozen in liquid nitrogen and be revived by putting them quickly back into regular water. ) Check around how big a goldfish could be, and how long you can put the goldfish in liquid nitrogen, before you can't revive it anymore. Good luck!
I don't know if this is entirely true, because many people believe that in the future they will have a cure to bring them back, so many people pay to freeze themselves (or even just their heads!) I watched this documentary, and they put a frog in liquid nitrogen, and because of it's cell structure, it came back to life once they let it thaw. I will try to find you the documentary.

Yours truly,
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Old 01-25-2006, 09:17 AM
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Default Liquid Nitrogen

Any idea where you can find Liquid Nitrogen? My teacher says that it is dangerous, and I agree. You can't just buy it at a drug store!

Yours truly,
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Old 01-25-2006, 09:37 AM
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Any idea where you can find Liquid Nitrogen? My teacher says that it is dangerous, and I agree. You can't just buy it at a drug store!

Yours truly,
i guess ur kind of cracy if you want to do something like that just tell me i can help you but is really difficult ...
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Old 01-25-2006, 11:49 AM
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I don't think you can get liquid nitrogen if your teacher doesn't get it for you. (Try your school lab technician if you have one. You should have it if you ever use liquid nitrogen in a lab.) Check some school science companies, such as california and try sigma. I'm not sure if noncertified ppl could order it, though...

Good luck!
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