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Old 09-26-2008, 11:51 PM
aselin19920331 aselin19920331 is offline
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Question Shark antibodies

Hi, I'm doing this project on shark's immune system, and I have done some reading. This project involves a lot of Biotechnology. The following is my abstract. (or part of it)

Sharks have a highly efficient immune system. They have special immunoglobulins called IgNARs. These vary from human antibodies primarily because they have 2 complementarity determining regions, as opposed to the 6 CDRs found in humans.

CDRs are highly variable loops found at the ends of the antibody structure that recognize the antigens.

My hypothesis:

Considering the fact that Shark IgNARs have less CDRs than human immunoglobulins, are sharks' IgNARs structured better to identify and bind antigens?

I have done some searching on PubMed, and here's a link that has the description on how to approach the procedures and testing:
[URL="http://www.ncbi.nlm.nih.gov/pubmed/12919318?ordinalpos=3&itool=EntrezSystem2.PEntrez. Pubmed.Pubmed_ResultsPanel.Pubmed_DefaultReportPan el.Pubmed_RVDocSum"]http://www.ncbi.nlm.nih.gov/pubmed/12919318?ordinalpos=3&itool=EntrezSystem2.PEntrez. Pubmed.Pubmed_ResultsPanel.Pubmed_DefaultReportPan el.Pubmed_RVDocSum[/URL]

My main problem is that jargon is littered throughout this article. I need someone who can understand it to explain it to me. I understand that this this VNAR is exposed to the outer membrane translocase receptor mitochondria (TOM 70) which acts like an antigen, and that it produced an antigen-specific clone (VNAR 12F-11). The rest about expressing it in E.Coli puzzles me. I know what protein filtration is and I know what ELISA and BIAcore is, but the rest I just can't put together.

Can someone who's professional in this field explain the process to me? Thank you so much.
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ignar , immune system , sharks

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