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Old 10-05-2005, 02:32 PM
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Exclamation E Coli

After adding an ampicillin resistant plasmid to E Coli, is it possible to take the plasmid back out and make the e coli non-resistant again?
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Old 10-06-2005, 01:31 AM
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Default Bacteria Transformation

If plasmids and Ecoli are your cup of tea, I have just the project for you! See [url]http://www.all-science-fair-projects.com/project867_78.html[/url]


as for your question, I've not tried that myself, but will my colleagues if they know. It would be interesting to find out - although I suspect that the answer is no - ie: once the bacteria acquires the resistance, it remains resistant.
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Old 10-08-2005, 02:42 AM
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I agree with you Mad Scientist well I'm not realy good at this area but it is hard I think it is very unlikely that it will be resistanced again.
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