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-   -   bacteria or botany? (http://classroom.all-science-fair-projects.com/forum/showthread.php?t=2450)

mcdoh1902 10-08-2006 03:50 PM

bacteria or botany?
 
Hi.

I'm supposed to have a question centered in the life sciences (biology) and I was wondering if anyone could help me.

If I were to do a project using bacteria, where can I get the petri dishes and the gelatin? And how do I regulate what kind of bacteria I grow? Can I buy bacteria?

And if I were to grow plants, and cover their leaves with vaseline so they couldn't breathe, how does that relate to real life? Why is it important?

Those are the only two questions I have that are relevant to the life sciences as of now, so if anyone has any other suggestions, that'd be great. :)

EDIT: As of now, I am rather set on the question of "What is the effect that the tobacco mosaic virus has on mold?"

Mold is easy, petri dishes, well, I have lots of answers to that now, and I know you can get the tobacco mosaic virus from cigarettes and infected plants. It's nearing winter, and I'm pretty sure none of the hardware stores/gardener stores have any infected plants for sale, so any other sources? I know you can buy it, but you need a teacher, and I think they need a permit, and it's rather expensive. After all, they're expecting people with budgets larger than mine. :)

dreamerofeternity 10-08-2006 03:54 PM

Quote:

Originally Posted by mcdoh1902
Hi.

I'm supposed to have a question centered in the life sciences (biology) and I was wondering if anyone could help me.

If I were to do a project using bacteria, where can I get the petri dishes and the gelatin? And how do I regulate what kind of bacteria I grow? Can I buy bacteria?

And if I were to grow plants, and cover their leaves with vaseline so they couldn't breathe, how does that relate to real life? Why is it important?

Those are the only two questions I have that are relevant to the life sciences as of now, so if anyone has any other suggestions, that'd be great. :)

First of all, I suggest the bacteria lab. You can buy petri dishes (agar plates) from your school (your school can order them for you). You can take bacteria from the air, and select a colony of bacteria to transfer to a plate so you'd get a select strain of bacteria. But I believe you can buy bacteria.

Remember when growing bacteria, to keep STERILE! That means, every equipment must be burned in the fire before touching the agar plates/bacteria.


Good luck!

mcdoh1902 10-08-2006 03:59 PM

Quote:

Originally Posted by dreamerofeternity
First of all, I suggest the bacteria lab. You can buy petri dishes (agar plates) from your school (your school can order them for you). You can take bacteria from the air, and select a colony of bacteria to transfer to a plate so you'd get a select strain of bacteria. But I believe you can buy bacteria.

Remember when growing bacteria, to keep STERILE! That means, every equipment must be burned in the fire before touching the agar plates/bacteria.


Good luck!

Thanks. I forgot about having to sterilize the equipment to prevent contamination. Great advice.

dreamerofeternity 10-08-2006 04:01 PM

Quote:

Originally Posted by mcdoh1902
Thanks. I forgot about having to sterilize the equipment to prevent contamination. Great advice.

Well, to be a bit more specific, I was thinking, one of those small wires attached to a cork. You can burn it in an ethanol fuse, and wait for a second for it to cool (metal is easy to cool), then touch the colony you want, and touch that colony unto the new plate.

Good luck!

satfire311 10-09-2006 04:56 AM

???
 
To Sterilize, you coud also use ultraviolet lights. It is a little known method that scientists use. It what teachers use to clean off goggles. Although, you would have to ask your school where/if you could use them.

Hope this helps
Satfire311:note:

mcdoh1902 10-09-2006 05:36 PM

Thanks. I edited my question a bit now, to better fit what I have available, so instead of bacteria, now it's mold, but I'm still going to have to sterilize petri dishes. Would anyone have a thought to a good source of tobacco mosaic virus? My friend tried testing bacteria once, and she said nothing happened, although that could have been because she couldn't extract the virus.


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