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Mad Scientist 08-08-2005 02:07 AM

Trends in Microbiology
 
Anyone have ideas about current trends for science projects in the area of microbiology? One of our members would like to know what sort of project might be popular in this area.

wisteria 10-25-2005 06:04 AM

first mad what is mirobiology?

brainygirl 10-25-2005 02:14 PM

This might help wisteria!
 
Hi, Wisteria

Well microbiology can be the study of micro- organisms, that includes protozoans, algea, fungi, bactaria and viruses. Organisms that are to small for the human eyes to see moreover it can be organisms that can only be seen under a microscope. :)

brainygirl 10-25-2005 02:33 PM

This might help!!!
 
Determine the effect of incubation temperature on bacteria growth rate ?

Materials needed

4 Incubators
12 Plastic test tubes
18 Petri dishes
My bacteria
1 Moist plastic bag
Broth that supports the bacteria growth
A Data sheet
1 Pair of latex or vinyl gloves
1 Fluid impermeable lab coat
1 Nephlometer
2 Micro liter measurers (10 and 20 uLs)
1 Density measurer

Procedures

1) Take two Petri dishes and remove the lids

2) Set one of the dishes in a kitchen and one in bedroom

3) Let them collect bacteria for one hour

4) After an hour place the lids on the dishes

5) Place the dishes in a moist plastic bag and seal

6) Take to a hospital and have them analyze the bacteria and place each collected sample in a separate prepared broth that supports their growth

7) Let the bacteria grow enough so that you can work with it for your experiment

8) Divide the two cultures of bacteria into 2 tubes each and add more broth

9) Label those tubes 1-4
Conduct Temperature Trials:

10) Place 12 test tubes on a tray in 4 rows of 3.

11) Label the first tube in each row 1, 2, 3, 4.

12) Pump 1. 8 ml. distilled water into the first test tube in the 1st row

13) Take a cotton swab and remove some bacteria out of the #1 sample of bacteria.

14) Swish the swab around in the water of the first test tube in the 1st row

15) Measure and adjust turbidity of test tube contents on an electronic density measurer so that it has 80% light transmission.

16) Take 20 micro liters (uL) of the solution in the first test tube in the 1st row and place it in the second test tube of that same row

17) Add 1. 8 ml. distilled water to the second test tube.

18) Take 20 microliters (uL) of the solution in the second test tube in the 1st row and place it in the third test tube of that same row

19) Add 1. 8 ml. distilled water to the third test tube.

20) Repeat steps 12-19 to the remaining 3 rows, except use only the respective bacteria for that row (for example, bacteria sample #3 is only used for test tubes in row #3)

21) Sort 16 sterile Petri dishes into groups of 4 and label each group with a number, 1-4. Within each group additionally label each dish with one of four temperatures to be tested: 42 C. , 37 C. , 20 C. , 5 C.

22) From the third test tube of row #1 draw 10 uL of the diluted bacteria and place into a Petri dish labeled #1. Do this four times total, once for each temperature group: 42, 37, 20, 5.

23) Spread the bacteria around the plate in 3 directions (vertically, horizontally, and diagonally) with a plastic loop.

24) Repeat steps 22 and 23 with the diluted bacteria from the other 3 rows being sure to place bacteria in the respectively numbered Petri dishes.

25) Place the Petri dishes in the four incubators at the temperatures specified on each dish.

26) Leave them in the incubator for 6 days

27) Take the Perti dishes out immediately after that time

28) Count the live bacteria colonies with the use of a magnifying glass (if
necessary) or the unaided eye.

29) Record the number on a data sheet

30) After all the bacteria tests have been done kill all the bacteria you have worked with. They must be autoclaved. :)

wisteria 10-27-2005 05:26 AM

thanks brainygirl and that is a good experinment I'll try it when I have time which I don't :( ... and keep up the good work...

BroadwayBaby 11-04-2005 03:53 PM

Microbiology Rocks!!
 
This is kind of off task, but I just love micro biology. It has got to be my favorite branch of science. Besides in the medical feild, what types of career involve microbiology?

ScienceIsCool 11-04-2005 04:08 PM

Quote:

Originally Posted by BroadwayBaby
This is kind of off task, but I just love micro biology. It has got to be my favorite branch of science. Besides in the medical feild, what types of career involve microbiology?

Many fields involve microbiology. There are companies that are developing drugs to cure all sorts of diseases (such as cancer). These require expertise in micro biology.

Also, there is an entire field of engineering/science called bio-engineering. These days, a lot of the cutting edge research is done at the micro level - for example, to figure out how to physically manipulate cells, or how to measure certain signals (such as level of electricity etc) at the cell level -- basically, how to "interface" man made devices with human cells. One of the goals of bio engineering is to help cure diseases or fix all sorts of problems inside the human body.

Mad Scientist 11-04-2005 04:45 PM

Great project
 
Brainygirl, thanks for the excellent suggestion for the microbiology project. Did you do this project yourself? 1f you did, I'm going to feature this under the Featured Projects section. Simply Fantastic!

BroadwayBaby 11-05-2005 11:02 AM

Microbiology Careers
 
Quote:

Originally Posted by ScienceIsCool
Many fields involve microbiology. There are companies that are developing drugs to cure all sorts of diseases (such as cancer). These require expertise in micro biology.

Also, there is an entire field of engineering/science called bio-engineering. These days, a lot of the cutting edge research is done at the micro level - for example, to figure out how to physically manipulate cells, or how to measure certain signals (such as level of electricity etc) at the cell level -- basically, how to "interface" man made devices with human cells. One of the goals of bio engineering is to help cure diseases or fix all sorts of problems inside the human body.

Whoa, thanx ScienceIsCool, I'll check those out!

wisteria 11-05-2005 09:53 PM

I still don't know what I would like to do :eek: and my parents are pestering me and I'm just in grade/ year 9...huh... well it sounds cool maybe maybe... :confused:

BroadwayBaby 11-06-2005 01:19 PM

Sticky?
 
This is also sort of off topic, but what does the STICKY mean at the begining of the thread title??

sciencefreak 11-19-2005 06:43 AM

Well could somebody gimme some info on biotechnology, biochemistry, genetic engineering and clinical research. I just can't choose among these careers for myself coz i'm definitely going for science later.

Mad Scientist 11-20-2005 07:49 AM

Stickies
 
Quote:

Originally Posted by BroadwayBaby
This is also sort of off topic, but what does the STICKY mean at the begining of the thread title??

This means that the thread is stuck. It will stay at the top of the list of threads in this particular forum, regardless of how old this thread is. As for normal (non-sticky) threads, they get pushed down the list as time goes by. :) We should stick threads that are good, meaningful, and well discussed (with lots of substance). Give it a go!

SmartyC 12-21-2005 03:09 PM

project for microbiology
 
do mcro organisms effect the plants growth?

first get some soil and bake in the oven until you think the micro organisms have gone. plant a plant in that soil you baked.

Get some soil that hasn't been baked or tampered with. plant a plant in that soil the same time as the other one.

watch which one grows more and determine if microrganisms effect the growth in any way.

p.s. make sure that you water them the same and give them each equal sun light.

science ace #1 01-03-2006 01:58 PM

Another Microbiology Project!
 
you can try to see what kind of bacteria lie on the skin and then see which are susceptible to an antibiotic like penicillin

it's a really good project.

and i also love microbiology. it's the best!

luv 2 help!!:) :)

satfire311 01-03-2006 02:01 PM

Not impressed
 
Well, not to be rude, but microbiology doewsn't interest me that much at all. It seemes really boring. But if other members like it them maybe Mad could make a forum for it.

BroadwayBaby 01-21-2006 09:43 AM

That bio-engineering thing sounds really interesting... Can I get more details????? By the way, onion cells are really neat looking!

GuyLazenby 10-30-2008 11:57 PM

Trends in Microbiology
 
hi,


That bio-engineering thing sounds really interesting... Can I get more details????? By the way, onion cells are really neat looking!:angel:


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